AMCA PHLEBOTOMY 2 LATEST VERSIONS
ACTUAL EXAM WITH 250 QUESTIONS AND
CORRECT DETAILED ANSWERS (VERIFIED
ANSWERS) [ALREADY GRADED A+]
How does Sodium Citrate work? - Sodium Citrate prevents coagulation by
binding to calcium in the specimen. It is the additive used for coagulation
studies because it preserves the coagulation factors in the specimen. It is a
liquid additive that requires specific blood to additive ratio: 9 parts blood to
1 part additive.
*Remember: Sodium Citrate is the additive in the Light Blue Top Tube.
True or False: The light-blue top tube must be filled completely to avoid an
inadequate ratio of additive to blood. - True; it must be drawn to the fill line.
2 | P a g e
For the Light Blue Top Tube, after the sample is collected, how many times
must it be inverted? - It must be inverted 3-4 times to mix blood and
additive properly.
True or False: Current CLSI standards mandate for the phlebotomist to
draw a red top tube before a light blue top tube, even though the requisition
does not call for it. - True
Why do current CLSI standards mandate for the phlebotomist to draw a red
top tube before a light blue top tube, even though the requisition does not
call for it? - This is to prevent thromboplastin released by the tissue cells
during venipuncture from entering the light-blue tube or to clear the line of
air when using a winged infusion set. Both thromboplastin and air can
affect the coagulation study results.
What is the order of draw? - Sterile Bottles --> Blue --> Red --> Yellow -->
Green --> Purple --> Gray
*Note: If Sterile Bottles are NOT drawn, the Red Top Tube will be drawn
before the Blue Top Tube.
3 | P a g e
List the tubes with anticoagulants. - Lavender, Green, Pink, Royal Blue with
a Light Blue stripe, Light Blue, and Gray.
List the tubes without anticoagulants. - Red, Tiger Top, Gold tubes,
"speckled" tubes, and Royal Blue with a Red stripe.
Tubes with anticoagulants produce __________ when separated in a
centrifuge. - plasma
Tubes without anticoagulants produce __________ when separated in a
centrifuge. - serum
Collecting specimens in the incorrect order of draw can result in
__________? - Cross-contamination
What is the approved order of draw? - 1) Sterile Bottles
2) Light Blue Top Tube
3) Red Top Tube
4) Tiger top, speckled tube or Gold Top Tube
5) Mint Green Top Tube
4 | P a g e
6) Dark Green Top Tube
7) Lavender or Light/Dark Purple
8) Pink Top Tube
9) Gray Top Tube
10) Royal Blue Top Tube
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